Reducing cellular dysfunction caused by mitochondrial gene mutations
AssigneeUniversity of Florida Research Foundation, Inc.
InventorJohn Guy
Life of this patent
Estimating the term…
Abstract
A synthetic, nuclear-encoded ND4 gene was linked to a mitochondrial targeting sequence and a FLAG epitope tag. This fusion construct was inserted into a rAAV vector. The ND4 fusion protein was expressed and imported into the mitochondria of cells harboring a mitochondrial DNA mutation (G11778A), where it restored cellular respiration.
Claims11
An isolated nucleic acid comprising a nucleotide sequence encoding a functional ND4 mitochondrial protein, wherein the nucleotide sequence comprises the sequence of SEQ ID NO:1.
An isolated cell into which has been introduced a nucleic acid comprising a nucleotide sequence encoding a functional ND4 mitochondrial protein wherein the nucleotide sequence comprises the sequence of SEQ ID NO:
- 1.
Dependencies are inferred from the claim text.