US 7,405,267 B2Granted

Method for purifying teicoplanin A2

AssigneeCKD Bio Corp.

InventorsTae-Won Kang, Byoung-Taek Choi, Gang-Sun Choi, Yong-Rack Choi, Sung-Ho Hwang

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Abstract

Disclosed is a method for purifying teicoplanin A2 comprising: (i) a primary pre-purification step of purifying a filtrate of fermentation broth of a strain using a synthetic adsorbent; (ii) a secondary pre-purification step of purifying the primary pre-purification solution using a cation exchange resin having a high cross-linkage, a catalytic resin or a chelate resin; (iii) a final purification step of purifying the secondary pre-purification solution using a reversed phase resin; and (iv) a powder-forming step. According to the present invention, it is possible to obtain teicoplanin A2 with a higher purity through a relatively simple process without using an excessive amount of an organic solvent.

Claims8

Dependent claims

A method for purifying teicoplanin A2 comprising:

(i) purifying a filtrate of a fermentation broth comprising teicoplanin A2 on a synthetic polymeric adsorbent, to obtain a primary purification solution;
(ii) purifying the primary purification solution on a cation exchange resin having a cross-linkage of over 8% resin;
a chelate resin;
or a resin selected from the group consisting of acidic porous resins comprising a styrene polymer matrix, acidic cation exchange resins comprising a porous styrene polymer matrix, and gel-type acidic polymer resins, to create a secondary purification solution;
(iii) purifying the secondary purification solution on a reversed phase resin, to create a tertiary teicoplanin A2 purification solution;
and
(iv) drying the tertiary teicoplanin A2 purification solution to form a powder.

4 more elements

Dependencies are inferred from the claim text.