# US 7,405,202 B2 · CRISP polypeptides as contraceptives and inhibitors of sperm capacitation

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- Number: US 7,405,202 B2
- Document ID: US7405202B2
- Status: Granted
- Kind: B2
- Application number: 10/515,868
- Filed: 2003-05-28
- Published: 2005-12-22
- Granted: 2008-07-29
- Earliest claimed: 2002-05-28
- Assignees: Regents of the University of Minnesota
- Inventors: David W. Hamilton; Kenneth P. Roberts; Kathy M. Ensrud
- Web page: https://patentgrep.com/patent/US7405202B2

## CPC

- C07K 14/47: from mammals
- A61K 38/1709: {from mammals}
- A61P 15/16: Masculine contraceptives
- A61P 15/18: Feminine contraceptives
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS

## Abstract

Included in the present invention are methods of inhibiting sperm capacitation, inhibiting the phosphorylation of a protein at tyrosine residues, inhibiting an acrosomal reaction, and inhibiting fertilization of an egg by sperm with the administration of a CRISP polypeptide.

## Claims

### Claim 1

A method of inhibiting sperm capacitation comprising contacting sperm that have not undergone capacitation with a cysteine-rich secretory protein (CRISP) polypeptide under capacitation conditions, wherein the CRISP polypeptide has at least 95% sequence identity to human CRISP-1 (SEQ ID NO: 1) and the CRISP polypeptide inhibits tyrosine phosphorviation of sperm proteins.

### Claim 2

A method of inhibiting sperm capacitation comprising contacting sperm that have not undergone capacitation with a cysteine-rich secretory protein (CRISP) polypeptide under capacitating conditions, wherein the CRISP polypeptide is human CRISP-1 (SEQ ID NO:1).

### Claim 3

A method of inhibiting sperm capacitation comprising contacting sperm with the human CRISP-1 polypeptide SEQ ID NO: 1.