Method and apparatus for purifying and desalting biological samples
AssigneeBecton, Dickinson and Company
InventorXiaoxi (Kevin) Chen
Life of this patent
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Abstract
The subject invention provides a sample processing technique for purifying a biological or chemical sample. The invention is particularly well-suited to prepare a sample for mass spectrometry. The process is to be performed in an article having at least one well, in which the surface of the well is at least partially hydrophobic and/or modified with bio-specific ligands. Targeted solutes, such as salts or small molecule contaminants, can be removed from a solution to allow for a purified solution of a desired type of solute, such as peptides and/or proteins.
Claims25
A process for purifying a biological or chemical sample, said process comprising:
- providing an article having a well with a surface which is at least partially hydrophobic, said well having an open end and a closed end;
- depositing a liquid sample in said well, said liquid sample being a solution including liquid and first and second classes of solutes;
- evaporating said liquid from said liquid sample such that said first and second classes of solutes come out from solution from said liquid sample, said first class of solutes in said sample tightly binding to said surface, and said second class of solutes in said sample not tightly binding to said surface;
- depositing a first buffer in said well, said first buffer disassociating said second class of solutes from said surface;
- removing said first buffer and said disassociated second class of solutes from said well;
- depositing a second buffer in said well after removing said first buffer, said second buffer disassociating said first class of solutes from said surface.
Dependencies are inferred from the claim text.