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US 7,405,082 B2Granted
Methods and devices for measuring reticulocytes
AssigneeSysmex Corporation
InventorsToshihiro Mizukami, Yusuke Mori, Kanako Horisaka, Sunao Atogami
Life of this patent
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Abstract
Methods and devices are described for monitoring a therapeutic effect in a red blood cell-related disease by detecting the size of a reticulocyte using a full-automatic blood cell counting device.
Claims12
A method for measuring a reticulocyte comprising:
- (a) preparing a measurement sample by mixing a reticulocyte measurement reagent and a whole blood sample from a subject;
- (b) introducing the measurement sample into a flow cell of a flow cytometer;
- (c) irradiating cells in the measurement sample flowing in the flow cell with an excitation light;
- (d) detecting scattering light and fluorescent light emitted from the cells;
- (e) making a two-dimensional distribution diagram having two axes corresponding to forward scattering light intensity and fluorescent light intensity, respectively;
- (f) specifying a mature red blood cell region and a reticulocyte region in the two-dimensional distribution diagram;
- (g) calculating a first mean forward scattering light intensity in the reticulocyte region;
- (h) calculating a second mean forward scattering light intensity by performing (a) through (g) using a whole blood sample taken from the subject after a period of time and after administration of a therapeutic agent for anemia to the subject;
- (i) detecting a difference between the first and the second mean forward scattering light intensities;
- and
- (j) outputting monitoring information of a therapeutic effect of the agent on anemia based on the difference.
8 more elements
A method for measuring a reticulocyte comprising:
- (a) preparing a measurement sample by mixing a reticulocyte measurement reagent and a whole blood sample;
- (b) introducing the measurement sample into a flow cell of a flow cytometer;
- (c) irradiating cells in the measurement sample flowing in the flow cell with an excitation light;
- (d) detecting scattering light and fluorescent light emitted from the cells;
- (e) making a two-dimensional distribution diagram having two axes corresponding to forward scattering light intensity and fluorescent light intensity, respectively;
- (f) specifying a mature red blood cell region and a reticulocyte region in the two-dimensional distribution diagram;
- (g) dividing the reticulocyte region into a plurality of regions on the basis of the fluorescent light intensity;
- and
- (h) calculating a mean forward scattering light intensity and a mean fluorescent light intensity for each of the plurality of regions.
6 more elements
A method for measuring a reticulocyte comprising:
- (a) preparing a measurement sample comprising a reticulocyte measurement reagent and a first whole blood sample;
- (b) introducing the measurement sample into a flow cell of a flow cytometer;
- (c) irradiating cells in the measurement sample flowing in the flow cell with an excitation light;
- (d) detecting scattering light and fluorescent light emitted from the cells;
- (e) making a two-dimensional distribution diagram having two axes corresponding to forward scattering light intensity and fluorescent light intensity, respectively;
- (f) specifying a mature red blood cell region and a reticulocyte region in the two-dimensional distribution diagram;
- (g) calculating a mean forward scattering light intensity and a mean fluorescent light intensity in the mature red blood cell region and the reticulocyte region;
- and
- (h) calculating a first slope of a first linear regression line representing a relationship between the mean forward scattering light intensity and the mean fluorescent light intensity in the mature red blood cell region and the reticulocyte region.
6 more elements
A method for measuring a reticulocyte comprising:
- (a) preparing a measurement sample comprising a reticulocyte measurement reagent and a whole blood sample;
- (b) introducing the measurement sample into a flow cell of a flow cytometer;
- (c) irradiating cells in the measurement sample flowing in the flow cell with an excitation light;
- (d) detecting scattering light and fluorescent light emitted from the cells;
- (e) making a two-dimensional distribution diagram having two axes corresponding to forward scattering light intensity and fluorescent light intensity, respectively;
- (f) specifying a mature red blood cell region and a reticulocyte region in the two-dimensional distribution diagram;
- (g) calculating a mean forward scattering light intensity in the mature red blood cell region and a mean forward scattering light intensity in the reticulocyte region;
- (h) plotting data obtained in (g) on a graph having two axes corresponding to the forward scattering light intensity in the mature red blood cell region and the forward scattering light intensity in the reticulocyte region, respectively;
- and
- (i) calculating distance of the data plotted in (h) from a datum point predetermined on the graph.
7 more elements
A reticulocyte measurement device comprising:
- a flow cell for receiving a measurement sample that comprises a reticulocyte measurement reagent and a whole blood sample;
- a light source for irradiating cells in the measurement sample with an excitation light;
- a first detector for detecting intensity of scattering light emitted from the cells irradiated with the excitation light;
- a second detector for detecting intensity of fluorescent light emitted from the cells irradiated with the excitation light;
- and
- an analysis unit for making a two-dimensional distribution diagram having two axes corresponding to scattering light intensity and fluorescent light intensity, for specifying a mature red blood cell region and a reticulocyte region in the two-dimensional distribution diagram, for dividing the reticulocyte region into a plurality of regions on the basis of the fluorescent light intensity, and for calculating a mean forward scattering light intensity and a mean fluorescent light intensity for each of the plurality of regions.
3 more elements
A reticulocyte measurement device comprising:
- a flow cell for receiving a measurement sample that comprises a reticulocyte measurement reagent and a whole blood sample;
- a light source for irradiating cells in the measurement sample with an excitation light;
- a first detector for detecting intensity of scattering light emitted from the cells irradiated with the excitation light;
- a second detector for detecting intensity of fluorescent light emitted from the cells irradiated with the excitation light;
- and
- an analysis unit for making a two-dimensional distribution diagram having two axes corresponding to scattering light intensity and fluorescent light intensity, for specifying a mature red blood cell region and a reticulocyte region in the two-dimensional distribution diagram, for calculating a mean forward scattering light intensity and a mean fluorescent light intensity for each of the mature red blood cell region and the reticulocyte region, and for calculating slope of a linear regression line representing a relationship between the mean forward scattering light intensity and the mean fluorescent light intensity.
3 more elements
A reticulocyte measurement device comprising:
- a flow cell for receiving a measurement sample that comprises a reticulocyte measurement reagent and a whole blood sample;
- a light source for irradiating cells in the measurement sample with an excitation light;
- a first detector for detecting intensity of scattering light emitted from the cells irradiated with the excitation light;
- a second detector for detecting intensity of fluorescent light emitted from the cells irradiated with the excitation light;
- and
- an analysis unit for making a two-dimensional distribution diagram having two axes corresponding to scattering light intensity and fluorescent light intensity, for specifying a mature red blood cell region and a reticulocyte region in the two-dimensional distribution diagram, for calculating a mean forward scattering light intensity in the mature red blood cell region and a mean forward scattering light intensity in the reticulocyte region, for plotting data on a graph having two axes corresponding to forward scattering light intensity in the mature red blood cell region and forward scattering light intensity in the reticulocyte region, and for calculating distance of the plotted data from a datum point predetermined on the graph.
3 more elements
Dependencies are inferred from the claim text.