Compositions and methods to reduce the complexity of transgene integration into the genome of a plant
AssigneePioneer Hi-Bred International, Inc.
InventorsChristopher L. Baszczynski, Benjamin A. Bowen, David J. Peterson, Laura Tagliani
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Abstract
Methods for the targeted integration of nucleotide sequences into a plant are provided. Transfer cassettes comprising nucleotide sequences of interest flanked by non-identical recombination sites are used to transform a plant comprising a target site. The target site contains at least a set of non-identical recombination sites corresponding to those on the transfer cassette. Exchange of the nucleotide sequences flanked by the recombination sites is effected by a recombinase.
Claims23
A method for reducing the complexity of integration of transgenes in a genome of a plant cell, said method comprising:
- a) introducing into the plant cell a transfer cassette flanked by a first and a second recombination site, wherein said first and said second recombination sites are non-identical, wherein said transfer cassette further comprises a nucleotide sequence of interest and, wherein said genome of the plant cell comprises a target site comprising the first and the second non-identical recombination sites of said transfer cassette, wherein said first and said second non-identical recombination sites can recombine with their identical recombination site;
- b) providing a recombinase that recognizes and implements recombination at the non-identical recombination sites and thereby allows for the directional targeting of a nucleotide sequence of interest at the target site;
- and,
- c) selecting the plant cells having reduced complexity of integrated transgenes in their genome, wherein said reduced complexity comprises the integration of the transfer cassette at the target site.
Dependencies are inferred from the claim text.