Bed material for cell culture, method for co-culture of cell and co-cultured cell sheet obtainable therefrom
AssigneeCellseed Inc.
InventorsTeruo Okano, Masayuki Yamato, Akihiko Kikuchi
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Abstract
By using a bed material for cell culture having a surface composed of two domains of domain A coated with a temperature-responsive polymer and domain B composed of any one or a combination of a domain coated with a polymer having high affinity with cells, a domain coated with the temperature-responsive polymer in an amount different from the amount of the temperature-responsive polymer of domain A, and a domain coated with a polymer which responds to a temperature different from the temperature to which domain A responds, a method for the co-culture of a plurality of kinds of cells which has heretofore been difficult becomes possible.
Claims9
A bed material for cell culture having an array of one or more domains of domain A coated with a temperature-responsive polymer and one or more domains of domain B composed of any one or a combination of any two or three of (1) a domain coated with a polymer having high affinity with cells, (2) a domain coated with the temperature-responsive polymer in an amount different from the amount of the temperature-responsive polymer of domain A, with the difference in the amount coated between domains A and B being at least 0.10 μg/cm2 or more, and (3) a domain coated with a polymer which responds to a temperature different from the temperature to which domain A responds.
Dependencies are inferred from the claim text.