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US 7,405,059 B2Granted
Modified Shine-Dalgarno sequences and methods of uses thereof
AssigneeHuman Genome Sciences, Inc.
InventorMichael W. Laird
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Abstract
Novel Shine-Dalgarno (ribosome binding site) sequences, vectors containing such sequences, and host cells transformed with these vectors are provided. Methods of use of such sequences, vectors, and host cells for the efficient production of proteins and fragments thereof in prokaryotic systems are also provided. In particular embodiments of the invention, compounds and methods for high efficiency production of soluble protein in prokaryotic systems are provided.
Claims10
A method of producing B. anthracis protective antigen polypeptide comprising:
- (a) generating a vector comprising a Shine-Dalgarno sequence selected from a group consisting of:
- (i) SEQ ID NO:2;
- (ii) polynucleotides 4-13 of SEQ ID NO:2;
- and
- (iii) SEQ ID NO:18;
- operably associated with a polynucleotide encoding B. anthracis protective antigen polypeptide;
- (b) generating a recombinant host cell comprising said vector;
- (c) culturing said recombinant host cell under conditions suitable to produce B. anthracis protective antigen polypeptide;
- and
- (d) recovering B. anthracis protective antigen polypeptide from the cell culture.
7 more elements
Dependencies are inferred from the claim text.