Method of extracting nucleic acid or protein using dendrimers and dendrimer-compositional substances
AssigneeYokogawa Electric Corporation
InventorsTadashi Matsunaga, Haruko Takeyama, Brandon Yoza, Kazuhisa Fukushima, Saya Satou
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Abstract
The present invention relates to a method of extracting nucleic acid or protein, in which multi-layer dendrimers are formed on the surface of fine particles, amino radicals for capturing nucleic acid or protein are formed on the surface of these dendrimers, and nucleic acid or protein is extracted using these amino radicals. The present invention can greatly and easily increase the recovery ratio of nucleic acid or protein.
Claims29
A method of extracting nucleic acid or protein using dendrimers, comprising:
- extracting nucleic acid or protein from a fluid using amino radicals of particulate bodies mixed and dispersed in the fluid containing the nucleic acid or protein,
- wherein the particulate bodies comprise multi-layer dendrimers formed on the surface of fine particles, the amino radicals being formed on the surface of the dendrimers.
A fluid containing dendrimers-compositional substances mixed and dispersed in the fluid, wherein the dendrimers-compositional substances are composed of particulate bodies comprising multi-layer dendrimers repeatedly synthesized on the surface of fine particles, and amino radicals covering the surface of the above dendrimers, said particulate bodies being capable of being mixed and dispersed in a fluid containing nucleic acid or protein and configured so that nucleic acid or protein can be captured by these amino radicals.
A method of extracting nucleic acid or protein using dendrimers in which multi-layer dendrimers formed on the surface of fine particles, amino radicals are formed on the surface of the dendrimers, and nucleic acid or protein is extracted using these amino radicals, wherein said fine particles are magnetic bodies.
A method of extracting nucleic acid or protein using dendrimers in which multi-layer dendrimers formed on the surface of fine particles, amino radicals are formed on the surface of the dendrimers, and nucleic acid or protein is extracted using these amino radicals, wherein said fine particles have a size of about 50 to about 60 microns.
Dendrimers-compositional substances which are composed of fine particles, multi-layer dendrimers repeatedly synthesized on the surface of these fine particles, and amino radicals covering the surface of the above dendrimers, and are configured so that nucleic acid or protein can be captured by these amino radicals, wherein said fine particles are magnetic bodies.
Dendrimers-compositional substances which are composed of fine particles, multi-layer dendrimers repeatedly synthesized on the surface of these fine particles, and amino radicals covering the surface of the above dendrimers, and are configured so that nucleic acid or protein can be captured by these amino radicals, wherein said fine particles have a size of about 50 to about 60 microns.
Dependencies are inferred from the claim text.