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US 7,405,037 B2Granted
Methods and tools for detecting collagen degradation
AssigneeLonza Walkersville, Inc.
InventorDale Greenwalt
Life of this patent
Estimating the term…
Abstract
Fluorophore-labeled collagen covalently bound to a cell culture vessel can be used to assay collagen degradation by a variety of cell types, including osteoclasts and tumor cells. Such assays provide a high throughput platform for rapid screening of large numbers of potential modulators of, for example, tumor metastasis or osteoclast differentiation and/or function.
Claims20
A method of detecting collagen degradation, comprising the steps of:
- (a) culturing cells in culture medium on fluorophore-labeled collagen covalently bound to a culture surface of a cell culture vessel, wherein the cells can degrade the collagen or can differentiate into cells which can degrade the collagen;
- (b) detecting the presence or absence of a fluorescence signal in a sample of the culture medium, wherein fluorescence signal intensity reflects the concentration of fluorophore-labeled collagen fragments in the sample of the culture medium.
A method of detecting collagen degradation, comprising the steps of:
- (a) culturing cells selected from the group consisting of osteoclasts and osteoclast precursors in culture medium on europium chelate-labeled human collagen type I covalently bound to a culture surface of a cell culture vessel;
- (b) transferring a sample of the culture medium from the cell culture vessel to an assay vessel;
- and
- (c) detecting the presence or absence of a fluorescence signal in the assay vessel, wherein fluorescence signal intensity reflects the concentration of europium chelate-labeled collagen fragments in the sample of the culture medium.
1 more element
A method of detecting collagen degradation, comprising the steps of:
- (a) culturing human osteoclasts in culture medium on europium chelate-labeled human collagen type I covalently bound to a culture surface of a cell culture vessel, wherein the europium chelate is Eu3+-N′-(p-isothiocyanatobenzyl) diethylenetriamine-N1,N2, N3-tetraacetic acid;
- (b) transferring a sample of the culture medium from the cell culture vessel to an assay vessel;
- and
- (c) detecting the presence or absence of a fluorescence signal in the assay vessel, wherein fluorescence signal intensity reflects the concentration of europium chelate-labeled collagen fragments in the sample of the culture medium.
1 more element
Dependencies are inferred from the claim text.