Induced activation in dendritic cell
AssigneeBaylor College of Medicine
InventorsDavid Spencer, Brent Hanks, Kevin Slawin
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Abstract
The present invention is directed to a composition and method which to treat diseases and to enhance a regulated immune response. More particularly, the present invention is drawn to compositions that are based on dendritic cells modified to express an inducible form of a co-stimulatory polypeptide.
Claims52
A method for activating an antigen presenting cell, which comprises:
- transducing an antigen presenting cell in vitro or ex vivo with a nucleic acid having a nucleotide sequence that encodes a chimeric protein, wherein the chimeric protein comprises a myristoylation membrane targeting region, a FK506 ligand-binding region and a CD40 cytoplasmic polypeptide region lacking the CD40 extracellular domain;
- and
- contacting the antigen presenting cell with a multimeric FK506 or FK506 analog ligand that binds to the FK506 ligand-binding region;
- whereby the antigen presenting cell is activated.
A composition which comprises a nucleic acid having a polynucleotide sequence that encodes a chimeric protein, wherein the chimeric protein comprises a myristoylation membrane targeting region, a FK506 ligand-binding region that can bind to a FK506 and/or FK506 analog molecule, and a CD40 cytoplasmic polypeptide region lacking the CD40 extracellular domain.
A method for inducing an immune response against an antigen, which comprises
- transducing an antigen presenting cell in vitro or ex vivo with a nucleic acid having a nucleotide sequence that encodes a chimeric protein, wherein the chimeric protein comprises a myristoylation membrane targeting region, a FK506 ligand-binding region, and a CD40 cytoplasmic polypeptide region lacking the CD40 extracellular domain;
- contacting the antigen presenting cell with an antigen ex vivo or in vitro;
- contacting the antigen presenting cell with a multimeric FK506 or FK506 analog ligand that binds to the FK506 ligand-binding region;
- and
- contacting the antigen presenting cell with a T-cell;
- whereby an immune response against the antigen is induced.
A method for inducing an immune response against an antigen in vivo, which comprises administering to a subject by a propelling force a composition that includes particles, a nucleotide sequence encoding a chimeric protein and a nucleotide sequence encoding an antigen,
- wherein the chimeric protein comprises a myristoylation membrane targeting region, a FK506 ligand-binding region that can bind to a FK506 and/or FK506 analog molecule and a CD40 cytoplasmic polypeptide region lacking the CD40 extracellular domain, and
- whereby an immune response is induced against the antigen.
Dependencies are inferred from the claim text.