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US 7,404,930 B2Granted
Method and system for performing in continuous flow a biological, chemical or biochemical protocol
AssigneeCommissariat a l'Energie Atomique
InventorsYves Fouillet, Raymond Charles, Nicolas Sarrut, Patricia Claustre
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Abstract
The invention concerns the performing, in continuous flow, of a biological, chemical or biochemical protocol on substances to be analysed, comprising several steps which consists in: in causing a mobile analysis support (11) comprising means (12) for receiving substances to be analysed and reagents to move past; implementing the steps of the protocol on the substances as the mobile analysis support (11) moves past.
Claims27
A system to perform a biological, chemical or biochemical protocol in continuous flow on substances to be analysed comprising:
- a mobile analysis support comprising means of reception of substances to be analysed and reagents, wherein the reception means are arranged so as to form a matrix of rows and columns;
- a mechanism connected to the support, where the mechanism moves with a continuous movement or in small steps thereby enabling each column in the reception means to pass in front of one or more different elements necessary to perform the protocol;
- a liquid film covering the reception means, where the liquid film is immiscible with the substances to be analysed and the reagents, thereby preventing the evaporation of the substances to be analysed and the reagents;
- means of delivering the substances to be analysed to the reception means,
- means of delivering the reagents to the reception means;
- and
- heating means arranged such that, while the protocol is being carried out, each column in reception means passes in front of the relevant heating means and delivery means, the arrangement of heating means and delivery means being set up in accordance with said protocol, thereby bringing the reception means to different determined temperatures successively, wherein
- the means of delivering the reagents is between the means of delivering the substances to be analysed and the heating means.
5 more elements
A system to perform a biological, chemical or biochemical protocol in continuous flow on substances to be analysed comprising:
- a mobile analysis support comprising means of reception of substances to be analysed and reagents, wherein the reception means are arranged so as to form a matrix of rows and columns;
- a mechanism connected to the support, where the mechanism moves with a continuous movement or in small steps thereby enabling each column in the reception means to pass in front of one or more different elements necessary to perform the protocol;
- a liquid film covering the reception means, where the liquid film is immiscible with the substances to be analysed and the reagents, thereby preventing the evaporation of the substances to be analysed and the reagents;
- means of delivering the substances to be analysed to the reception means,
- means of delivering the reagents to the reception means;
- and
- heating means arranged such that while the protocol is being carried out each column in the reception means passes in front of the heating means after passing in front of the means of delivering the reagents, thereby bringing the reception means to different determined temperatures successively, wherein
- the means of delivering the substances to be analysed is between the means of delivering the reagents and the heating means.
5 more elements
A system to perform a biological, chemical or biochemical protocol in continuous flow on substances to be analysed comprising:
- a mobile analysis support comprising means of reception of substances to be analysed and reagents, wherein the reception means are arranged so as to form a matrix of rows and columns;
- a mechanism connected to the support, where the mechanism moves with a continuous movement or in small steps thereby enabling each column in the reception means to pass in front of one or more different elements necessary to perform the protocol;
- a liquid film covering the reception means, where the liquid film is immiscible with the substances to be analysed and the reagents, thereby preventing the evaporation of the substances to be analysed and the reagents;
- means of delivering the reagents to the reception means;
- heating means arranged such that while the protocol is being carried out each column in the reception means passes in front of the heating means after passing in front of the means of delivering reagents, thereby bringing the reception means to different determined temperatures successively;
- and
- detection means for the substances on which the protocol has been performed, thereby providing an analysis of these substances, wherein
- the heating means is between the means of delivering the reagents and the detection means.
5 more elements
Dependencies are inferred from the claim text.