Flow cytometry system with bubble detection
AssigneeAccuri Cytometers, Inc.
InventorCollin Rich
Life of this patent
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Abstract
The flow cytometry system of the present invention includes a flow channel including an interrogation zone. A light source and a light detector are connected to the interrogation zone, such that a sample flowing through the interrogation zone can be optically analyzed through methods known in the art of flow cytometry. A bubble detector is connected to the flow channel. A controller is connected to the bubble detector and is adapted to perform a predetermined output in response to the detection of a bubble in the flow channel. The predetermined output may include alerting a user as to the presence of a bubble, flagging potentially corrupted data, and ceasing data collection until the interrogation zone is clear of bubbles.
Claims19
A flow cytometry system for collecting data on a sample fluid, comprising:
- a flow channel adapted to contain and direct a sample fluid, the flow channel defining an interrogation zone;
- a light source connected to the interrogation zone and adapted to emit light toward the sample fluid in the interrogation zone;
- a light detector connected to the interrogation zone and adapted to collect light from the interrogation zone and to provide collection data based on the collected light;
- a bubble detector coupled to the flow channel and adapted to detect the presence of a bubble in the flow channel;
- and
- a controller connected to the light detector and to the bubble detector and adapted to flag data within the collection data provided by the light detector during the detection of a bubble in the flow channel by the bubble detector.
flow cytometry system for collecting data on a sample fluid, comprising:
- a flow channel adapted to contain and direct a sample fluid, the flow channel defining an interrogation zone;
- a light source connected to the interrogation zone and adapted to emit light toward the sample fluid in the interrogation zone;
- a light detector connected to the interrogation zone and adapted to collect light from the interrogation zone;
- a bubble detector coupled to the flow channel and adapted to detect the presence of a bubble in the flow channel;
- and
- a controller connected to the bubble detector and adapted to cease data collection by controlling the light source and the light detector.
A flow cytometry system for collecting data on a sample fluid, comprising:
- a flow channel adapted to contain and direct a sample fluid, the flow channel defining an interrogation zone;
- a bubble detector coupled to the flow channel and adapted to detect the presence of a bubble in the flow channel;
- a sheath fluid pump adapted to pump sheath fluid from a sheath fluid container into the flow channel;
- and
- a controller connected to the bubble detector and adapted to cease data collection by controlling the sheath fluid pump.
Dependencies are inferred from the claim text.