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US 7,402,734 B2Granted
Method and apparatus for preparation of genetically transformable plant tissue
AssigneeMonsanto Technology LLC
InventorsBrian J. Martinell, Beth Jo Calabotta, Richard J. Heinzen, Richard F. Klemm, Dennis E. McCabe, Gail A. Roberts, Lori Ann Smith
Life of this patent
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Abstract
A process of mechanical separation of embryos from seeds for genetic transplantation employs counter-rotating cylinders together with one or more culling, hydration, separation, and viability testing steps to provide high-throughput of viable, transplantable tissue.
Claims37
A method of bulk preparation of transformable plant tissue comprising the steps of:
- (a) collecting plant seeds having a predetermined hydration;
- (b) passing the plant seeds through a mechanical separator to divide the seeds into a separate cotyledon, seed coat and embryo;
- and (c) transforming the separated embryo though an introduction of genetic material into cells of the separated embryo.
A method for the automated isolation of transformable plant tissue from a batch of seeds comprising the steps of:
- collectively passing a batch of seeds through a mechanical separator to isolate a stream of transformable plant tissue from said batch of seeds;
- and transforming the isolated transformable plant tissue by introducing genetic material into cells of said transformable plant tissue.
Dependencies are inferred from the claim text.