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US 7,402,732 B2Granted
Paraquat resistance gene and a vascular tissue—and trichome-specific promoter
AssigneesToyota Jidosha Kabushiki Kaisha, Okayama Prefecture
InventorsHisashi Ito, Yoshikazu Tanaka, Kenichi Ogawa, Satoshi Kondo, Chikara Ohto, Iwao Furusawa
Life of this patent
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Abstract
A paraquat resistance gene and a vascular tissue- and trichome-specific promoter are provided. The paraquat resistance gene and the vascular tissue- and trichome-specific promoter are isolated by identifying and analyzing genes of Arabidopsis thaliana.
Claims15
A method for screening for a transgenic plant, comprising
- introducing a recombinant vector into a plant, wherein the recombinant vector comprises a gene encoding a protein of the following (a) or (b):
- (a) a protein comprising the amino acid sequence represented by SEQ ID NO:
- 2;
- (b) a protein consisting of an amino acid sequence having a substitution, deletion or addition of one to ten amino acids relative to the amino acid sequence represented by SEQ ID NO:
- 2 and capable of imparting paraquat resistance;
- and wherein the recombinant vector further comprises a foreign gene or a foreign DNA fragment;
- and
- screening for a transgenic plant on the basis of paraquat resistance as an indicator.
5 more elements
A method for imparting paraquat resistance to a plant, comprising
- introducing a recombinant vector into a plant, wherein the recombinant vector comprises a gene encoding a protein of the following (a) or (b):
- (a) a protein comprising the amino acid sequence represented by SEQ ID NO:
- 2;
- (b) a protein consisting of an amino acid sequence having a substitution, deletion or addition of one to ten amino acids relative to the amino acid sequence represented by SEQ ID NO:
- 2 and capable of imparting paraquat resistance;
- imparting paraquat resistance;
- and
- confirming paraquat resistance.
5 more elements
A method for screening for a transgenic plant, comprising
- introducing a recombinant vector into a plant, wherein the recombinant vector comprises a gene encoding a protein of the following (a) or (b):
- (a) a protein comprising the amino acid sequence represented by SEQ ID NO:
- 2;
- (b) a protein consisting of an amino acid sequence having a substitution, deletion or addition of one to ten amino acids relative to the amino acid sequence represented by SEQ ID NO:
- 2 and capable of imparting paraquat resistance;
- and
- screening for a transgenic plant on the basis of paraquat resistance as an indicator.
4 more elements
Dependencies are inferred from the claim text.