Loading patent…
AssigneeKirin Pharma Kabushiki Kaisha
InventorsYoshimi Kuroiwa, Kazuma Tomizuka, Hitoshi Yoshida, Isao Ishida
Estimating the term…
The present invention relates to a human artificial chromosome which is genetically transmissible to the next generation with high efficiency and the method for using the same. More specifically, the present invention relates to: a human artificial chromosome in which an about 3.5 Mb to about 1 Mb region containing an antibody λ light chain gene derived from human chromosome 22 is bound to a chromosome fragment which is transmissible to a progeny through a germ line of a non-human animal, said chromosome fragment is derived from another human chromosome; a non-human animal carrying the human artificial chromosome and an offspring thereof; a method for producing the non-human animal; a method for producing a human antibody using the nonhuman animal or an offspring thereof; and a human antibody-producing mouse carrying the human artificial chromosome.
A chimeric human antibody-producing mouse, wherein the antibody-producing mouse carries (1) human artificial chromosome ΔHAC or ΔΔHAC in which a 2.5 Mb region (ΔHAC) or a 1.5 Mb region (ΔΔHAC) from human chromosome 22 containing an unrearranged human antibody λ light chain locus is linked to a human chromosome 14 fragment containing an unrearranged human antibody heavy chain locus and (2) an unrearranged human antibody κ light chain transgene retained on KCo5 that is inserted into a mouse chromosome of the human antibody-producing mouse, at least both alleles of endogenous antibody heavy chain and κ light chain are disrupted or inactivated, and a human antibody heavy chain comprising a human antibody Ig γ isotype, a human antibody κ light chain, and a human antibody λ light chain are expressed in serum;
Dependencies are inferred from the claim text.