Method for preparing a Centella asiatica extract rich in madecassoside and in terminoloside
AssigneeBayer Consumer Care AG
InventorsAlain Loiseau, Gérard Sene, Eric Theron
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Abstract
The invention concerns a method for preparing an extract comprising a mixture of madecassoside, terminoloside and optionally asiaticoside, an extract of Centella asiatica comprising more than 75 wt % of a mixture of madecassoside, terminoloside and asiaticoside, relative to the extract total weight, an extract of Centella asiatica comprising more than 95 wt. % of a mixture of madecassoside and terminoloside relative to the extract total weight and their use for regulating inflammatory mechanisms.
Claims9
A method for preparing an extract of Centella asiatica containing a mixture of madecassoside, terminoloside and asiaticoside comprising the steps of:
- a) creating an extract by exposing the parts of Centella asiatica that are above ground to an alcoholic solvent to obtain an alcoholic solution;
- b) passing the alcoholic solution obtained in step a), over an anionic resin to obtain an aqueous-alcoholic eluate;
- c) selectively defatting the aqueous-alcoholic eluate obtained in step b) by liquid/liquid extraction to obtain a defatted aqueous-alcoholic phase;
- d) concentrating the defatted aqueous-alcoholic phase obtained in step c) using successive filtration to obtain an aqueous phase;
- e) passing the aqueous phase obtained in step d), over a cationic resin and then over an anionic resin;
- and
- f) stabilizing the aqueous phase obtained in step e) by adding alcohol to obtain a mixture comprising madecassoside, terminoloside and asiaticoside.
A method for preparing an extract of Centella asiatica comprising a mixture containing madecassoside and terminoloside comprising the steps of:
- a) creating an extract by exposing the parts of Centella asiatica that are above ground by to an alcoholic solvent to obtain an alcoholic solution;
- b) passing the alcoholic solution obtained in step a), over an anionic resin to obtain an aqueous-alcoholic eluate;
- c) selectively defatting the aqueous-alcoholic eluate obtained in step b) by liquid/liquid extraction to obtain a defatted aqueous-alcoholic phase;
- d) concentrating the defatted aqueous-alcoholic phase obtained in step c) using successive filtration to obtain an aqueous phase;
- e) passing the aqueous phase obtained in step d), over a cationic resin and then over an anionic resin;
- f) stabilizing the aqueous phase obtained in step e) by adding alcohol to obtain a prepurified aqueous-alcoholic phase;
- g) separating the prepurified aqueous-alcoholic phase obtained in step f) using a chromatographic column having a stationary phase and a mobile phase;
- and
- h) obtaining a mixture comprising madecassoside and terminoloside.
Dependencies are inferred from the claim text.