Use of nanoparticles for the DNA administration to a target organ
AssigneeNanoDel Technologies GmbH
InventorsBernhard Sabel, Christian Walz, Kerstin Ringe
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Abstract
The invention relates to the use of DNA-loaded nanoparticles for a transfection of DNA into cells in vitro or in vivo.
Claims39
A method for transfecting DNA into cells in vivo, the method comprising contacting the cells with a polyalkylcyanoacrylate nanoparticle loaded with the DNA, whereby the DNA enters the cells.
A method for administering DNA to a target organ in a subject, the method comprising:
- (a) preparing polyalkylcyanoacrylate nanoparticles by polymerizing one or more alkylcyanoacrylates;
- (b) loading said polyalkylcyanoacrylate nanoparticles with DNA;
- and
- (c) administering said DNA-loaded polyalkylcyanoacrylate nanoparticles to the subject in an amount sufficient to obtain an effective concentration of the DNA in the target organ.
A method for administering DNA to a target organ in a human or animal body, the method comprising contacting the target organ with a polyalkylcyanoacrylate nanoparticle loaded with DNA, whereby the DNA enters one or more cells of the target organ.
A method for suppressing tumor cell proliferation, the method comprising contacting a tumor cell with a DNA-loaded polyalkylcyanoacrylate nanoparticle, whereby the DNA enters the tumor cell and suppresses proliferation of the tumor cell.
A method of target-focused treatment of a human or an animal to prevent and/or therapeutically treat cancer, the method comprising administering to the human or the animal an effective amount of one or more DNA sequences effective against cancer or encoding a polypeptide effective against cancer, wherein said DNA sequences are loaded onto one or more polyalkylcyanoacrylate nanoparticles.
Dependencies are inferred from the claim text.