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US 7,402,432 B2Granted
Process for producing T lymphocytes
AssigneeThe Brigham and Women's Hospital, Inc.
InventorsRachael A. Clark, Thomas Kupper
Life of this patent
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Abstract
The present invention is directed to an in vitro method for producing T lymphocytes. The method involves culturing bone marrow cells on a matrix seeded with keratinocytes and fibroblasts.
Claims26
A method for producing T lymphocytes in vitro for administration to a patient, comprising:
- (a) removing a skin sample from said patient;
- (b) separately culturing keratinocytes and fibroblasts derived from said skin sample;
- (c) colonizing a support matrix with the cultured keratinocytes and fibroblasts of step (b);
- (d) obtaining hematopoietic precursor cells from the bone marrow of said patient;
- (e) adding said bone marrow hematopoietic precursor cells to the colonized matrix of step (c);
- (f) culturing said bone marrow hematopoietic precursor cells on said support matrix in the presence of one or more factors selected from the group consisting of IL-15;
- IL-7;
- and Flt-3 ligand, so as to induce production of T lymphocytes;
- and
- (g) harvesting T lymphocytes from said matrix.
7 more elements
A method for producing T lymphocytes in vitro for administration to a patient who has undergone transplantation of an organ donated by a donor, said method comprising:
- (a) removing a skin sample from said donor;
- (b) separately culturing keratinocytes and fibroblasts derived from said skin sample;
- (c) colonizing a support matrix with the cultured keratinocytes and fibroblasts of step (b);
- (d) obtaining hematopoietic precursor cells from the bone marrow of said patient;
- (e) adding bone marrow hematopoietic precursor cells to the colonized matrix of step (c);
- (f) culturing said bone marrow hematopoietic precursor cells on said support matrix in the presence of one or more factors selected from the group consisting of IL-15;
- IL-7;
- and Flt-3 ligand, so as to induce production of T lymphocytes;
- and
- (g) harvesting T lymphocytes from said matrix.
7 more elements
Dependencies are inferred from the claim text.