MHC class II antigen-presenting systems and methods for activating CD4+ T cells
AssigneeThe Scripps Research Institute
InventorsSusan R. Webb, Ola Winqvist, Lars Karlsson, Michael R. Jackson, Per A. Peterson
Life of this patent
Estimating the term…
Abstract
The present invention relates to synthetic antigen-presenting matrices, their methods of making and their methods of use. One such matrix is cells that have been transfected to produce MHC antigen-presenting molecules with one or more accessory molecules. The matrices are used to activate naive CD4+ T cells as well as shift the ongoing activation state into a preferred differentiated population of either Th1 or Th2 cells.
Claims11
A method of producing an insect synthetic antigen presenting cell (APC) comprising:
- a) providing a cell lacking a gene encoding at least one of MHC class II α-chain, MHC class II β-chain, and an accessory molecule;
- and
- b) transforming the cell with an expressible gene for each of the genes of (a) lacking in the cell, the gene being operably linked to a promoter in a vector capable of expressing the gene.
A method of producing an insect synthetic antigen presenting cell (APC) comprising:
- a) providing a cell lacking a gene encoding at least one of MHC class II α-chain, MHC class II β-chain, an accessory molecule and an antigen processing assisting molecule;
- and
- b) transforming the cell with an expressible gene for each of the genes of (a) lacking in the cell, the gene being operably linked to a first operable promoter in a vector capable of expressing the gene.
Dependencies are inferred from the claim text.