Apparatus for the detection of pepsin
AssigneeBiomed Solutions, LLC
InventorsFarhan Taghizadeh, Scott Horner, Kevin Bucholtz
Life of this patent
Estimating the term…
Abstract
An apparatus for detecting pepsin comprising a solid support, and a peptide chain wherein the peptide chain is operatively configured to be cleaved by pepsin, and the peptide chain is disposed on a surface of the solid support.
Claims44
An article for detecting the presence of pepsin suitable to test a subject in a physician's office, the subject suspected of suffering from gastroesophageal reflux disease and providing a sample suspected of containing pepsin, the article comprising:
- a. a solid support comprising a test area surface;
- b. a multiplicity of peptide chains each having a peptide proximal end and a peptide distal end,
- wherein the peptide distal end comprises a peptide bearing a first tag that interacts with a second tag to provide a detectable chromophoric product,
- wherein all of said peptide proximal ends are attached to said test area surface of said support, and
- wherein said peptide chains are cleavable by said pepsin to provide said peptide proximal end attached to said test area surface and said peptide distal end bearing the detectable first tag cleaved from said test area surface.
An apparatus for detecting the presence of pepsin in a sample of material suitable to test a subject in a physician's office, the subject suspected of suffering from gastroesophageal reflux disease and providing the sample, said apparatus comprising:
- a. an article for exposure to said sample of material, said article comprising a substrate having a test area surface and a multiplicity of peptide chains each having a peptide proximal end and a peptide distal end,
- wherein the peptide distal end comprises a peptide bearing a detectable first tag,
- wherein all of said peptide proximal ends are attached to said test area surface of said substrate, and
- wherein said peptide chains are cleavable by said pepsin to provide said peptide proximal end attached to said test area surface and said peptide distal end bearing the detectable first tag cleaved from said test area surface;
- and
- b. a container comprising a fillable volume for receiving a test solution comprising at least one of the sample, a buffer and a second tag that interacts with the first tag to provide a detectable chromophoric product, and wherein at least the test area of said article is insertable in said fillable volume.
Dependencies are inferred from the claim text.