Recombinant methods and materials for producing epothilone and epothilone derivatives
AssigneeKosan Biosciences, Inc.
InventorsBryan Julien, Leonard Katz, Chaitan Khosla, Li Tang, Rainer Ziermann
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Abstract
Recombinant nucleic acids that encode all or a portion of the epothilone polyketide synthase (PKS) are used to express recombinant PKS genes in host cells for the production of epothilones, epothilone derivatives, and polyketides that are useful as cancer chemotherapeutics, fungicides, and immunosuppressants.
Claims15
A recombinant host cell that produces epothilone D and expresses proteins with the activity of the epothilone polyketide synthase proteins encoded in nucleotides 1998-56600 of SEQ. ID NO. 2, but does not express a protein with the epoxidase activity of the protein encoded in the epoK gene of nucleotides 56757-58016 of SEQ. ID NO. 2, wherein said cell produces more epothilone C than epothilone A and/or epothilone D than epothilone B.
A Sorangium cellulosum cell that does not express a gene product with the epoxidase activity of the protein encoded in the epoK gene of nucleotides 56757-58016 of SEQ. ID NO. 2, and wherein said cell produces more epothilone C than epothilone A and/or more epothilone D than epothilone B.
Dependencies are inferred from the claim text.