Back to results
US 7,402,419 B2Granted
Phosphite dehydrogenase mutants for nicotinamide cofactor regeneration
AssigneesBiotechnology Research and Development Corporation, The Board of Trustees of the University of Illinois
InventorsHuimin Zhao, William W. Metcalf, Wilfred A. van der Donk, Tyler Johnannes, Ryan Woodyer
Life of this patent
Estimating the term…
Abstract
Phosphite dehydrogenase mutant enzymes were generated that provide relaxed cofactor specificity and increased thermostability over the wild type enzyme. The mutant enzymes are useful for nicotinamide cofactor regeneration.
Claims7
A purified mutant of a wild-type phosphite dehydrogenase comprising the amino acid sequence of SEQ ID NO:
- 1, with improved catalytic activity for nicotinamide cofactor regeneration as compared with a wild-type phosphite dehydrogenase, and wherein the mutant phosphite dehydrogenase consists of an amino acid mutation selected from the group consisting of Glu175 to Ala 175 and Ala176 to Arg 176 of SEQ ID NO:
- 1.
A mutant of a wild-type phosphite dehydrogenase comprising the amino acid sequence of SEQ ID NO:
- 1, with improved thermostability and improved catalytic activity for nicotinamide cofactor regeneration as compared with a wild-type phosphite dehydrogenase and, wherein the mutant phosuhite dehydrogenase consists of one or more mutations selected from the group consisting of E175A;
- A176R;
- Q132R;
- Q137R;
- I150F;
- Q 215 L;
- R275Q;
- Q137R, I150F, Q215L, and R275Q;
- and Q132R, Q137R, I150F, Q215L, and R275Q of SEQ ID NO:
- 1.
7 more elements
Dependencies are inferred from the claim text.