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US 7,402,415 B2Granted
Method for the production of glycerol by recombinant organisms
AssigneesE.I. du Pont de Nemours and Company, Genencor International, Inc.
InventorsRamesh Nair, Mark S. Payne, Donald E. Trimbur, Fernando Valle
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Abstract
Recombinant organisms are provided comprising genes encoding a glycerol-3-phosphate dehydrogenase and/or a glycerol-3-phosphatase activity useful for the production of glycerol from a variety of carbon substrates. The organisms further contain disruptions in the endogenous genes encoding proteins having glycerol kinase and glycerol dehydrogenase activities.
Claims7
A method for the production of glycerol from a recombinant organism comprising:
- (i) transforming a suitable host cell with
- (a) an exogenous gene encoding a protein having glycerol-3-phosphate dehydrogenase activity, and
- (b) an exogenous gene encoding a protein having glycerol-3-phosphate phosphatase activity,
- the suitable host cell having a disruption in either one or both of
- (a) an endogenous gene encoding a polypeptide having glycerol kinase activity, wherein the disruption prevents the expression of the active gene product;
- and
- (b) an endogenous gene encoding a polypeptide having glycerol dehydrogenase activity, wherein the disruption prevents the expression of active gene product;
- (ii) culturing the transformed host cell of (i) in the presence of at least one carbon source selected from the group consisting of monsaccharides, oligosaccharides, polysaccharides, and single-carbon substrates, whereby glycerol is produced;
- and
- (iii) optionally recovering the glycerol produced in (ii), and wherein the suitable host cell is selected from the group consisting of bacteria, yeast and filamentous fungi.
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Dependencies are inferred from the claim text.