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US 7,402,405 B2Granted
Methods and kits for diagnosing a pancreatic-based fat malabsorption disorder
AssigneeThe Children's Hospital of Philadelphia
InventorVirginia A. Stallings
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Abstract
The present invention relates to methods and kits useful in the diagnosis of a pancreatic-based fat malabsorption disorder in a mammal.
Claims15
A method of diagnosing pancreatic-based fat malabsorption disorder in a mammal comprising the steps of:
- a) obtaining a baseline sample of blood from said mammal;
- b) administering to said mammal a digestible composition comprising a free fatty acid which can be absorbed from the small intestine of the mammal in the absence of pancreatic lipase hydrolysis thereof and a triglyceride which cannot be absorbed from the small intestine of the mammal in the absence of pancreatic lipase hydrolysis thereof;
- c) obtaining at least one subsequent sample of blood from said mammal following administration of said digestible composition;
- d) assaying said baseline sample and said at least one subsequent sample for said free fatty acid and the fatty acids of said triglyceride;
- e) determining from step d) the rate of fatty acid absorption of each of said free fatty acid and the fatty acids of said triglyceride in said mammal thereby obtaining a first absorption profile;
- and
- f) comparing the first absorption profile with a second profile of fatty acid absorption obtained after carrying out steps a), b) and c) on a control mammal being positive or negative for a pancreatic-based fat malabsorption disorder, whereby the presence of a pancreatic-based fat malabsorption disorder in said mammal is indicated when said first absorption profile is comparable to said second absorption profile obtained from a control mammal being positive for said pancreatic-based fat malabsorption and the absence of a pancreatic-based fat malabsorption disorder in said mammal is indicated when said first absorption profile is comparable to said second absorption profile obtained from a control mammal being negative for said pancreatic-based fat malabsorption disorder;
- wherein both said free fatty acid and said triglyceride are not radiolabeled;
- and
- wherein said free fatty acid has an odd number of carbons and said triglyceride comprises fatty acids having an odd number of carbons.
7 more elements
A kit for diagnosing a pancreatic-based fat malabsorption disorder in a mammal, said kit comprising
- a) an instructional material;
- b) a digestible composition consists of a first fatty acid-containing substrate which can be absorbed from the small intestine of the mammal in the absence of pancreatic lipase hydrolysis thereof and a second fatty acid-containing substrate which cannot be absorbed from the small intestine of the mammal in the absence of pancreatic lipase hydrolysis thereof, wherein said first fatty acid-containing substrate is selected from the group consisting of pentadecanoic acid (PA) and heptadecanoin (HA) and said second fatty acid-containing substrate is selected from the group consisting of tripentadecanoic acid (TPA) and triheptadecanoin (THA);
- and
- c) a tube or vial for obtaining a sample of blood from said mammal.
1 more element
Dependencies are inferred from the claim text.