Mammalian sweet taste receptors
AssigneesThe Regents of the University of California, The United States of America Department of Health and Human Services
InventorsCharles S. Zuker, Nicholas J. P. Ryba, Gregory A. Nelson, Mark A. Hoon, Jayaram Chandrashekar, Yifeng Zhang
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Abstract
The present invention provides isolated nucleic acid and amino acid sequences of sweet taste receptors comprising two heterologous G-protein coupled receptor polypeptides from the T1R family of sensory G-protein coupled receptors, antibodies to such receptors, methods of detecting such nucleic acids and receptors, and methods of screening for modulators of sweet taste receptors.
Claims13
A method of identifying a compound that activates or inhibits sweet taste signal transduction in taste cells, the method comprising the steps of:
- (i) contacting the compound with a cell expressing a sweet taste receptor comprising a T1R3 polypeptide and a T1R2 polypeptide, wherein the T1R3 polypeptide has a greater than 93% amino acid sequence identity to SEQ ID NO:
- 20 or greater than 93% amino acid sequence identity to SEQ ID NO:
- 23;
- and wherein the T1R2 polypeptide has a greater than 92% amino acid sequence identity to SEQ ID NO:8;
- and
- (ii) determining the functional effect of the compound upon the receptor, wherein said functional effect is binding to the receptor or having an effect on the activity of the receptor, thereby identifying a compound that activates or inhibits sweet signal transduction,
- wherein the sweet taste receptor specifically binds a sweet compound, and at least one of the T1R3 and T1R2 polypeptides is recombinant.
A method of identifying a compound that activates or inhibits sweet taste signal transduction in taste cells, the method comprising the steps of
- (i) contacting the compound with a cell expressing a sweet taste receptor comprising a T1R3 polypeptide and a T1R2 polypeptide, wherein the T1R3 polypeptide has a greater than 93% amino acid sequence identity to SEQ ID NO:20;
- and wherein the T1R2 polypeptide has a greater than 92% amino acid sequence identity to SEQ ID NO:8;
- and
- (ii) determining the functional effect of the compound upon the receptor, wherein said functional effect is binding to the receptor or having an effect on the activity of the receptor, thereby identifying a compound that activates or inhibits sweet signal transduction,
- wherein the sweet taste receptor specifically binds a sweet compound, and at least one of the T1R3 and T1R2 polypeptides is recombinant.
Dependencies are inferred from the claim text.