In vivo panning for ligands to radiation-induced molecules
AssigneeVanderbilt University
InventorsDennis E. Hallahan, Shimian Qu
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Abstract
A method for identifying a molecule that binds an irradiated tumor in a subject and molecules identified thereby. The method includes the steps of: (a) exposing a tumor to ionizing radiation; (b) administering to a subject a library of diverse molecules; and (c) isolating from the tumor one or more molecules of the library of diverse molecules, whereby a molecule that binds an irradiated tumor is identified. Also provided are therapeutic and diagnostic methods using targeting ligands that bind an irradiated tumor.
Claims22
A method for identifying a peptide that binds a radiation induced target in an irradiated tumor in a subject, the method comprising:
- (a) exposing a tumor in a subject to ionizing radiation;
- (b) administering to the subject a library of phage-displayed peptides wherein:
- (i)the phage-displayed peptides are 5-100 amino acids in length;
- and (ii) the administering step is performed about 4 hours to about 24 hours following he exposing step;
- (c) procuring from the subject the irradiated tumor or a fraction thereof;
- (d) isolating at least one phage from the procured irradiated tumor or from the a fraction thereof, wherein the at least one phage is bound to a radiation induced target in the procured irradiated tumor or the fraction thereof via a peptide displayed on its surface;
- and
- (e) identifying the peptide displayed on the surface of the phage that binds to the radiation-induced target in the irradiated tumor in the subject.
Dependencies are inferred from the claim text.