Method for detecting target plant genus

A method for detecting species in the genus Triticum in cases where even one species of the genus Triticum is contained in a subject to which the method is to be applied such as a food ingredient or product, which comprises the steps of:

• a) collecting 45S rRNA precursor gene sequences containing a ITS sequence of the genus Triticum and plants thought to be related species thereof and then selected the region common to the genus Triticum in the 45S rRNA precursor gene sequences;
• b) selecting the base specific to the genus Triticum from the region;
• c) designing primers (A) and/or (B) having the specific base at 3′ end thereof, which primers can hybridize under the stringent conditions to a nucleic acid molecule having a common nucleotide base sequence for all species in the genus Triticum in 45S rRNA precursor gene sequence thereof, wherein 3′ end of primer (A) can complementarily bind to a base in ITS-1 sequence of the genus Triticum when the primer hybridizes to the nucleic acid molecule while 3′ end of primer (B) can complementarily bind to a base in ITS-2 sequence of the genus Triticum when the primer hybridizes to the nucleic acid molecule;
• d) isolating DNA from the subject to which the method is to be applied;
• e) conducting PCR amplification for the isolated DNA using at least one member selected from the group consisting of the primers (A) and (B);
• and
• f) identifying the presence of the resulting amplification product from PCR containing at least a part of ITS-1 or ITS-2 sequence of the genus Triticum.