Method of immobilizing molecules onto a solid phase substrate and method of fabricating a biosensor using the method

A biosensor, comprising:

• a substrate;
• a plurality of first molecules that are immobilized to the substrate, each of the plurality of first molecules having a first functional portion that is used for recognizing a first object to be examined, and each of the plurality of first molecules including a first joint portion, the first functional portion being immobilized through the first joint portion that is capable of binding to the substrate;
• and
• a plurality of second molecules that are immobilized to the substrate, each of the plurality of second molecules having a second functional portion that is different from the first functional portion and that functions as a mediator of the first functional portion, and each of the plurality of second molecules including a second joint portion, the second functional portion being immobilized through the second joint portion that is capable of binding to the substrate.

A method of immobilization, comprising:

• immobilizing a plurality of first molecules and a plurality of second molecules to a solid phase substrate by putting a solution of the plurality of first and second molecules in contact with the solid phase substrate,
• each of the plurality of first molecules having a first functional portion that is used for recognizing a first object to be examined,
• each of the plurality of first molecules including a first joint portion, the first functional portion being immobilized through the first joint portion that is capable of binding to the solid phase substrate,
• each of the plurality of second molecules having a second functional portion that is different from the first functional portion,
• each of the plurality of second molecules including a second joint portion, the second functional portion being immobilized through the second joint portion that is capable of binding to the solid phase substrate, and
• the plurality of first molecules and the plurality of second molecules constituting a self-assembled monomolecular film.