Supercritical carbon dioxide extract of pharmacologically active components from Nerium oleander
AssigneePhoenix Biotechnology, Inc.
InventorCrandell Addington
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Abstract
A method for the extraction of pharmacologically active components from the oleander plant using supercritical carbon dioxide and combining the extraction with other isolation methods. The extraction is carried out using carbon dioxide at supercritical conditions, with or without the addition of a modifier. The isolated product is recovered in higher yield than with traditional hot water extraction.
Claims19
A method for extracting from powdered oleander leaf a pharmacologically active cardiac glycoside-containing extract useful in the treatment of cell proliferative disease said method comprising:
- mixing the powdered oleander leaf with a supercritical carbon dioxide with or without a modifier to give a supercritical solvent mixture, wherein the supercritical carbon dioxide is initially at a pressure above its critical pressure and a temperature above its critical temperature;
- decreasing the pressure and temperature of the supercritical solvent mixture to give a separated extract mixture;
- and
- recovering the pharmacologically active extract from the separated extract mixture to give the pharmacologically active cardiac glycoside-containing extract useful in the treatment of cell proliferative disease.
A method for extracting a pharmacologically active extract useful in the treatment of cell proliferative disease from powdered oleander leaf comprising:
- mixing the powdered oleander leaf with supercritical carbon dioxide with or without a modifier to give a supercritical solvent mixture, wherein the supercritical carbon dioxide is initially at a pressure of about 300 bar and a temperature of about 50° C;
- decreasing the pressure and temperature of the supercritical solvent mixture to give a separated extract mixture;
- and recovering the pharmacologically active extract from the separated extract mixture to give the pharmacologically active extract useful in the treatment of cell proliferative disease.
A method for extracting a pharmacologically active extract useful in the treatment of cell proliferative disease from powdered oleander leaf comprising:
- mixing the powdered oleander leaf with supercritical carbon dioxide to give a supercritical solvent mixture, wherein the supercritical carbon dioxide further comprises ethanol, and wherein the supercritical carbon dioxide is initially at a pressure of about 300 bar and a temperature of about 50° C;
- decreasing the pressure and temperature of the supercritical solvent mixture to give a separated extract mixture;
- and recovering the pharmacologically active extract from the separated extract mixture to give the pharmacologically active extract useful in the treatment of cell proliferative disease.
Dependencies are inferred from the claim text.