Cell-free tissue replacement for tissue engineering
AssigneeBoard of Regents, The University of Texas System
InventorsChristine Schmidt, Terry Hudson
Life of this patent
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Abstract
The present invention is a natural, cell-free tissue replacement that does not require difficult or extensive preparation made by washing tissue replacement in a solution including one or more sulfobetaines and an anionic surface-active detergent and washing the tissue replacement in serial solutions of the buffered salt to remove excess detergent. The natural, cell-free tissue replacement may be a nerve graft that supports axonal regeneration, guides the axons toward the distal nerve end and/or is immunologically tolerated. Other forms of the invention are a composition and kit prepared by the method of making a native, cell-free tissue replacement. The present invention may be modified for use in diagnostic, therapeutic, and prophylactic applications.
Claims18
A method for preparing a native, acellular nerve tissue replacement comprising the steps of:
- obtaining a nerve tissue;
- soaking the nerve tissue for at least six hours in a solution comprising one or more sulfobetaines;
- treating the nerve tissue in a mixture of one or more sulfobetaines and Triton X-200;
- and
- washing the nerve tissue in one or more solutions of a buffered salt to remove excess detergent to form the native, acellular nerve tissue replacement with significantly reduced immunologic response relative to a nerve tissue graft made acellular by a freeze/thaw process or a decellularization process utilizing Triton X-100.
A method for preparing a native, acellular nerve tissue replacement comprising the steps of:
- obtaining a nerve tissue;
- soaking the nerve tissue for at least six hours in a solution comprising one or more sulfobetaines;
- treating the nerve tissue in a mixture of one or more sulfobetaines and Triton X-200;
- and
- washing the nerve tissue in one or more solutions of a buffered salt to remove excess detergent to form the native, acellular nerve tissue replacement, wherein the basal laminae and endoneurium layer substantially retain the native extracellular matrix structure.
Dependencies are inferred from the claim text.