Method of delivering genes to the central nervous system of a mammal
AssigneesThe Trustees of the University of Pennsylvania, The Wistar Institute
InventorsJohn H. Wolfe, Nigel W. Fraser
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Abstract
The invention provides a method of delivering a selected DNA sequence to the central nervous system of a mammal by administering to said mammal a neurotropic virus, said virus containing a selected DNA sequence under the control of a promoter which permits expression of the DNA during the latent infectious state of the virus.
Claims8
A method of stably expressing a selected DNA sequence in the central nervous system of a mammal comprising administering to peripheral neuron cells of a mammal a neurotropic viral vector which infects cells of the central nervous system of the mammal, said vector containing a selected DNA sequence operatively linked to a LAT promoter so that said selected DNA sequence is stably expressed for at least four months by infected central nervous system cells.
A method of stably expressing β-glucuronidase in the brain of a mammal comprising administering to peripheral neuron cells of a mammal a neurotropic viral vector which infects cells of the brain of the mammal, said vector being an HSV-1 vector containing a DNA sequence encoding β-glucuronidase operatively linked to a LAT promoter, so that the infected brain cells stably express β-glucuronidase.
Dependencies are inferred from the claim text.