Method and compositions for promoting osteogenesis
AssigneeEnobia Pharma Inc.
InventorGuy Boileau
Life of this patent
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Abstract
The object of the present invention is to increase PHEX activity to improve the osteogenic process in a mammal where this process has been diminished due to pathology or a condition that require osteogenesis. Osteocalcin is an endogenous inhibitor of the PHEX activity. Therefore, the identification of a substance capable of potentiating PHEX activity by preventing the inhibitory action of endogenous inhibitors will improve osteogenesis. Since PHEX is generally associated with the growth plane of bone or teeth and the absence of osteocalcin is associated with increased bone mass, potentiation of PHEX activity can promote bone growth. Increased PHEX activity can also be obtained by administration of the enzyme itself.
Claims22
A method of treating a bone-related disorder requiring osteogenesis in a mammal, or treating a condition requiring osteogenesis in a mammal, comprising administering an effective amount of a soluble Phosphate Regulating Gene With Homologies To Endopeptidases On The X Chromosome protein (secPHEX) that comprises the sequence of amino acids as set forth in SEQ ID NO:11.
A composition for treating a bone-related disorder requiring osteogenesis in a mammal, or treating a condition requiring osteogenesis in a mammal comprising an effective amount of a soluble Phosphate Regulating Gene With Homologies To Endopeptidases On The X Chromosome protein (secPHEX) and a pharmaceutically-acceptable carrier, wherein the secPHEX protein comprises the sequence of amino acids as set forth in SEQ ID NO:11.
An orthopedic implant comprising a soluble Phosphate Regulating Gene With Homologies To Endopeptidases On The X Chromosome protein (secPHEX) protein that comprises the sequence of amino acids as set forth in SEQ ID NO:11.
A dental prosthesis comprising a soluble Phosphate Regulating Gene With Homologies To Endopeptidases On The X Chromosome protein (secPHEX) protein that comprises the sequence of amino acids as set forth in SEQ ID NO:11.
A method of producing a soluble Phosphate Regulating Gene With Homologies To Endopeptidases On The X Chromosome protein (secPHEX) comprising expressing an in frame fusion of a nucleotide sequence encoding the amino acid sequence from position 1 through position 63 of Neprilysin-Like peptidase (NL1) as set forth in SEQ ID NO:9 with a nucleotide sequence encoding the amino acid sequence set forth in SEQ ID NO:11.
A vector comprising an in frame fusion of a nucleotide sequence encoding the amino acid sequence from position 1 through position 63 of Neprilysin-Like peptidase (NL1) as set forth in SEQ ID NO:9 with a nucleotide sequence encoding the amino acid sequence set forth in SEQ ID NO:11.
A method of expressing a soluble Phosphate Regulating Gene With Homologies To Endopeptidases On The X Chromosome protein (secPHEX) in an isolated host cell comprising:
- (i) providing an expression vector comprising an in frame fusion of a nucleotide sequence encoding the amino acid sequence from position 1 through position 63 of Neprilysin-Like peptidase (NL1) set forth in SEQ ID NO:9 with a nucleotide sequence encoding the amino acid sequence set forth in SEQ ID NO:11;
- (ii) introducing the vector into an isolated host cell;
- and
- (iii) maintaining the isolated host cell under conditions permitting expression of secPHEX protein in the cell.
A method of separating a soluble Phosphate Regulating Gene With Homologies To Endopeptidases On The X Chromosome protein (secPHEX) from other components in a solution by applying the solution to a hydrophobic chromatographic column and removing those fractions comprising the secPHEX protein, wherein the secPHEX protein comprises the sequence of amino acids as set forth in SEQ ID NO:11, thereby separating the secPHEX protein from other components of the solution.
A method of separating a soluble Phosphate Regulating Gene With Homologies To Endopeptidases On The X Chromosome protein (secPHEX) from other components in a solution by applying the solution to an ion-exchange chromatographic column and removing those fractions comprising the secPHEX protein, wherein the secPHEX protein comprises the sequence of amino acids as set forth in SEQ ID NO:11, thereby separating the secPHEX protein from other components of the solution. fused at its amino terminus to the amino acid sequence set forth in SEQ ID NO:3.
Dependencies are inferred from the claim text.