Intravascular delivery of nucleic acid
AssigneeMiris Bio Corporation
InventorsJon A. Wolff, Vladimir G. Budker
Life of this patent
Estimating the term…
Abstract
Disclosed is a process for transfecting genetic material into a mammalian cell to alter endogenous properties of the cell. The process comprises designing a polynucleotide for transfection. Then the polynucleotide is inserted into a mammalian blood vessel. Prior to insertion, subsequent to insertion, or concurrent with insertion the permability of the vessel is increased using an exterior cuff and immunosuppression is applied thereby the genetic material is delivered to the parenchymal cell.
Claims25
A process for delivering a polynucleotide to a skeletal muscle cell of a mammal in vivo, comprising:
- a) applying non-invasive external pressure against the skin of a limb of the mammal such that blood flow to and from the limb is impeded;
- b) inserting the polynucleotide encoding a protein operably linked to a promoter in a solution into a blood vessel in the limb in vivo distal to the applied pressure;
- and,
- c) administering immunosuppressive drugs to the mammal;
- thereby delivering the polynucleotide to the skeletal muscle cell in the limb distal to the applied pressure and expressing the polynucleotide in the skeletal muscle cell at detectable levels.
A process for delivering a polynucleotide to a skeletal muscle cell of a mammal in vivo, comprising:
- a) applying pressure non-invasively against the skin of the limb thereby impeding blood flow into and out of the limb;
- b) inserting the polynucleotide encoding an expressible sequence operably linked to a promoter in a solution into a blood vessel in the limb of the mammal in vivo distal to the applied pressure;
- c) delivering the polynucleotides to the skeletal muscle cell of the limb distal to the applied pressure;
- and
- d) expressing the polynucleotide in the skeletal muscle cell to detectable levels;
- wherein said applying, said inserting, said delivering and said expressing do not diminish subsequent use of the limb by the mammal.
Dependencies are inferred from the claim text.