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US 7,396,811 B2Granted
Subcellular targeting of therapeutic proteins
AssigneeZyStor Therapeutics, Inc.
InventorsJonathan H. LeBowitz, Stephen M. Beverley
Life of this patent
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Abstract
Targeted therapeutics that localize to a specific subcellular compartment such as the lysosome are provided. The targeted therapeutics include a therapeutic agent and a targeting moiety that binds a receptor on an exterior surface of the cell, permitting proper subcellular localization of the targeted therapeutic upon internalization of the receptor. Nucleic acids, cells, and methods relating to the practice of the invention are also provided.
Claims14
A therapeutic fusion protein comprising:
- a lysosomal enzyme, and
- a mutein of mature human IGF-II that binds human cation-independent mannose-6-phosphate receptor in a mannose-6-phosphate-independent manner, wherein the mutein differs from mature human IGF-II only at a position selected from the group consisting of amino acid 9, amino acid 19, amino acid 26, and amino acid 27,
- wherein the mutein of mature human IGF-II is fused N-terminally to the lysosomal enzyme and wherein the therapeutic fusion protein is produced in a mammalian expression system using an IGF-II signal peptide and is therapeutically active in vivo.
A therapeutic fusion protein comprising:
- a lysosomal enzyme, and
- a mutein of mature human IGF-II that binds human cation-independent mannose-6-phosphate receptor in a mannose-6-phosphate-independent manner, wherein the mutein differs from mature human IGF-II only by a deletion or a replacement of amino acids 1-7,
- wherein the mutein of mature human IGF-II is fused N-terminally to the lysosomal enzyme and wherein the therapeutic fusion protein is produced in a mammalian expression system using an IGF-II signal peptide and is therapeutically active in vivo.
A therapeutic fusion protein comprising:
- a lysosomal enzyme, and
- a mutein of mature human IGF-II that binds human cation-independent mannose-6-phosphate receptor in a mannose-6-phosphate-independent manner, wherein the mutein differs from mature human IGF-II only by a deletion or a replacement of amino acids 62-67,
- wherein the mutein of mature human IGF-II is fused N-terminally to the lysosomal enzyme and wherein the therapeutic fusion protein is produced in a mammalian expression system using an IGF-II signal peptide and is therapeutically active in vivo.
A therapeutic fusion protein comprising:
- a lysosomal enzyme, and
- a mutein of mature human IGF-II that binds human cation-independent mannose-6-phosphate receptor in a mannose-6-phosphate-independent manner, wherein the mutein differs from mature human IGF-II only by a deletion or a replacement of amino acids 29-40,
- wherein the mutein of mature human IGF-II is fused N-terminally to the lysosomal enzyme and wherein the therapeutic fusion protein is produced in a mammalian expression system using an IGF-II signal peptide and is therapeutically active in vivo.
A therapeutic fusion protein comprising:
- a lysosomal enzyme, and
- a mutein of mature human IGF-II that binds human cation-independent mannose-6-phosphate receptor in a mannose-6-phosphate-independent manner, wherein the mutein differs from mature human IGF-II only by an amino acid substitution selected from the group consisting of Tyr27Leu, Leu43Val, and Ser26Phe,
- wherein the mutein of mature human IGF-II is fused N-terminally to the lysosomal enzyme and wherein the therapeutic fusion protein is produced in a mammalian expression system using an IGF-II signal peptide and is therapeutically active in vivo.
A therapeutic fusion protein comprising:
- a lysosomal enzyme, and
- a mutein of mature human IGF-II that binds human cation-independent mannose-6-phosphate receptor in a mannose-6-phosphate-independent manner, wherein the mutein differs from mature human IGF-II only by a deletion or a replacement of amino acids 1-7 and a substitution of Tyr27Leu,
- wherein the mutein of mature human IGF-II is fused N-terminally to the lysosomal enzyme and wherein the therapeutic fusion protein is produced in a mammalian expression system using an IGF-II signal peptide and is therapeutically active in vivo.
Dependencies are inferred from the claim text.