Method for cell adhesion and wound healing
AssigneeRegen Biotech, Inc.
InventorsIn-San Kim, Jung-Eun Kim
Life of this patent
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Abstract
The present invention relates to a method for cell adhesion and wound healing with internal domains of βig-h3. Particularly, the present invention relates to the method of using recombinant proteins comprising one or more of 2nd or 4th internal domain of βig-h3 for cell adhesion and wound healing, wherein the 2nd or 4th internal domain of βig-h3 has aspartic acid and isoleucine essential for interaction with integrin which represent a high homology in base sequence of βig-h3 internal domains. The recombinant proteins comprising one or more 2nd or 4th internal domain of βig-h3 are effective for cell adhesion and wound healing by itself and can be used for developing cell culture medium and wound healing agent.
Claims17
A method for inducing cell adhesion comprising coating a recombinant protein comprising one or more copies of the 4th fas-1 domain of βig-h3 onto a support and binding mammalian cells to the protein-coated support, wherein the recombinant protein does not include full-length βig-h3 protein.
A method for inducing cell adhesion comprising coating a recombinant protein comprising two or more copies of the 2nd and/or the 4th fas-1 domain of βig-h3 onto a support and binding mammalian cells to the protein-coated support.
A method for inducing cell adhesion comprising coating a recombinant protein consisting of one or more copies of the 2nd and/or the 4th fas-1 domain of βig-h3, alone or in combination, onto a support and binding mammalian cells to the protein-coated support.
A method for inducing cell adhesion comprising the steps of:
- 1) preparing a recombinant protein comprising one or more copies of the 4th fas-1 domain of βig-h3, by use of an expression vector, wherein the recombinant protein does not include full-length βig-h3 protein;
- 2) coating the recombinant protein onto a solid support;
- and
- 3) binding mammalian cells to the protein-coated solid support.
A method for healing a mammalian wound with a recombinant protein comprising one or more copies of the 2nd and/or the 4th fas-1 domain of βig-h3, wherein the recombinant protein does not include full-length βig-h3 protein, comprising the steps of:
- 1) coating a solid support with the recombinant protein comprising one or more copies of the 2nd and/or the 4th fas-1 domain of βig-h3;
- 2) binding mammalian cells to the protein-coated solid support;
- and
- 3) attaching said solid support to the wound.
Dependencies are inferred from the claim text.