Evanescent wave sensor with attached ligand
AssigneeAgilent Technologies, Inc.
InventorsKarla M. Robotti, Daniel B. Roitman
Life of this patent
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Abstract
The invention in particular embodiments provides an evanescent wave sensor which includes a ligand bound to a sensor substrate via a NCYX linker moiety, as defined herein. Methods of making the subject evanescent wave sensors are also provided which include contacting a first reactive moiety which has an isocyanato (or isothiocyanato) moiety with a second reactive moiety which has an hydroxyl, thiol, or amino moiety, as further described herein. Also provided by the invention are methods in which a subject evanescent wave sensor is contacted with a sample, and binding of analytes in the sample to the sensor is assessed by evanescent wave detection. The invention also provides kits and systems for performing the subject methods.
Claims25
A method of making a sensor, the method comprising
- contacting a first reactive moiety having the structure
- R1-N═C═Y1
- with a second reactive moiety having the structure
- R2-X1Hm
- under conditions sufficient to result in a compound having the structure
- wherein:
- one of R1 or R2 is a sensor substrate;
- the other of R1 or R2 is selected from a ligand, a linking group bound to a ligand, a functional group for binding a ligand, or a linking group bound to a functional group for binding a ligand;
- Y1 is selected from O or S;
- X1 is selected from N or S, provided that, if Y1 is S, X1 is N;
- n equals 1 when X1 is N, or n equals 0 when X1 is S;
- and
- m equals n plus 1.
A sensor having the structure:
- wherein:
- one of R1 or R2 is a sensor substrate;
- the other of R1 or R2 is selected from a ligand, a linking group bound to a ligand, a functional group for binding a ligand, or a linking group bound to a functional group for binding a ligand;
- Y1 is selected from O or S;
- X1 is selected from N or S, provided that, if Y1 is S, X1 is N;
- n equals 1 when X1 is N, or n equals 0 when X1 is S.
A system comprising:
- a sensor comprising the structure:
- wherein:
- one of R1 or R2 is a sensor substrate;
- the other of R1 or R2 is selected from a ligand, a linking group bound to a ligand, a functional group for binding a ligand, or a linking group bound to a functional group for binding a ligand;
- Y1 is selected from O or S;
- X1 is selected from N or S, provided that, if Y1 is S, X1 is N;
- and
- n equals 1 when X1 is N, or n equals 0 when X1 is S;
- and
- a light source.
Dependencies are inferred from the claim text.