Kit and method for determining a plurality of analytes

A kit for at least one of the simultaneous qualitative and quantitative determination of a multitude of analytes, said kit comprising:

• a single sensor platform comprising an optical thin-film waveguide with a first optically transparent layer and a second optically transparent layer, said first optically transparent layer being transparent at least at an excitation wavelength and being provided on said second optically transparent layer, said second optically transparent layer having a lower refractive index than said fist optically transparent layer, also being transparent at least at the excitation wavelength, and said sensor platform comprising at least one grating structure modulated in said first optically transparent layer for the incoupling of an excitation light into said first optically transparent layer;
• at least one array of biological, biochemical or synthetic recognition elements immobilized in discrete measurement areas directly or by an adhesion-promoting layer on said first optically transparent layer, for at least one of specific recognition binding of the analytes and specific interaction with the analytes;
• referencing means for laterally resolved referencing of the excitation light intensity available in the measurement areas, wherein the referencing means includes
• arrays of measurement areas arranged in segments of one or more measurement areas for analyte determination and of measurement areas for referencing,
• one or more arrays comprising segments of two or more measurement areas with biological or biochemical or synthetic recognition elements for analyte determination or for referencing, that are similar with each segment,
• two or more identical measurement areas provided for the determination of each analyte or for physical or chemical referencing within a segment or array,
• means for determining the background signal at the actual luminescence wavelength between or adjacent to the measurement areas, or
• luminescence marker spots comprising luminescently labeled molecules;
• calibration means for calibrating one or more luminescences generated in the near-field of said first optically transparent layer as a consequence of the binding of one or more analytes or of the specific interaction with one or more analytes,
• wherein a liquid sample to be analyzed for the analytes is brought into contact with the measurement areas on said sensor platform either directly or after mixture with further reagents.