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US 7,396,657 B2Granted
Lipase variants
AssigneeNovozymes A/S
InventorsSigne Munk, Jesper Vind, Kim Borch, Shamkant Anant Patkar, Sanne O. Schroder Glad, Allan Svendsen
Life of this patent
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Abstract
Attaching a peptide extension to the C-terminal amino acid of a lipase reduces the tendency to form odor. This may lead to lipase variants with a reduced odor generation when washing textile soiled with fat which includes relatively short-chain fatty acyl groups (e.g., up to C8) such as dairy stains containing butter fat or tropical oils such as coconut oil or palm kernel oil.
Claims27
A method of producing a polypeptide having lipase activity comprising:
- (a) culturing a cell comprising a nucleic acid sequence encoding a C-terminal extension linked to a nucleic acid sequence encoding a parent polypeptide having lipase activity, wherein the amino acid sequence of the C-terminal extension consists of 3-11 amino acids and wherein the amino acid at the first position is H, the amino acid at the second position is T, and the amino acid at the third position is P;
- and
- (b) recovering the polypeptide.
Dependencies are inferred from the claim text.