Method of cross-linking peptides
AssigneeAcademia Sinica
InventorsHan-Jia Lin, Yee-Hsiung Chen
Life of this patent
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Abstract
A method of cross-linking a peptide to form a homopolymer of the peptide, to immobilize the peptide on a solid phase and to enhance antigenicity of the peptide is disclosed. The method comprises the steps of preparing a fusion peptide by incorporating a cross-linking segment including a tetrapeptide sequence Q-X-K-(S/T) (SEQ ID NO: 15) into the peptide and cross-linking the peptide by a glutaminase.
Claims8
A method of cross-linking a peptide to form polymers of said peptide, comprising the steps of
- (a) incorporating a cross-linking segment comprising the Q-X-K-(S/T) sequence into said peptide to form a fusion polypeptide, wherein Q is glutamine, X is an amino acid having an aliphatic side chain, K is lysine and (S/T) is either serine or threonine;
- and
- (b) cross-linking said fusion polypeptide with a transglutaminase in a cross-linking reaction buffer to form said polymers.
A fusion polypeptide cross-linked with transglutaminase, wherein the fusion polypeptide comprises a first peptide fragment and a Q-X-K-(S/T) fragment of a different origin from said first peptide fragment, wherein Q is glutamine, X is an amino acid having an aliphatic side chain, K is lysine and (S/T) is either serine or threonine, said Q-X-K-(S/T) (SEQ ID NO:
- 15) fragment being incorporated into said first peptide fragment to form said fusion polypeptide.
A substrate for a transglutaminase, comprising a first peptide fragment and a Q-X-K-(S/T) fragment of a different origin from said first peptide fragment, wherein Q is glutamine, X is an amino acid having an aliphatic side chain, K is lysine and (S/T) is either serine or threonine, said Q-X-K-(S/T) fragment being incorporated into said first peptide fragment to form said substrate for a transglutaminase.
Dependencies are inferred from the claim text.