Method for enhancing activity of luciferase with fluorescence activity
AssigneeChisso Corporation
InventorsSatoshi Inouye, Satoko Sasaki
Life of this patent
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Abstract
Methods for enhancing luminescence of a luciferase (BFP-aq) with fluorescence activity derived from a calcium-binding photoprotein are provided. To a luciferase solution with fluorescence activity that contains an apoprotein, a calcium-binding photoprotein, which is constituted such that a coelenteramide or an analog thereof is coordinated inside, a coelenterazine that is the luminescent substrate of the luciferase or an analog thereof and a compound (e.g., imidazole etc.) having the function of removing an —NH-proton of the pyrazine ring of the imidazopyrazine skeleton in the coelenterazine or the analog thereof are added.
Claims12
A method for enhancing luminescence of a luciferase with fluorescence activity that contains an apoprotein in which a coelenteramide or an analog thereof is coordinated, comprising adding a compound selected from the group consisting of imidazole and Guanidine-HCl to a solution of the luciferase with the fluorescence activity and a coelenterazine or analog thereof and that is a luminescent substrate of the luciferase.
Dependencies are inferred from the claim text.