Back to results
US 7,396,653 B2Granted
Cell migration assay
AssigneeChemoCentryx, Inc.
InventorsZheng Wei, Zhenhua Miao
Life of this patent
Estimating the term…
Abstract
The present invention is directed to a modified cell migration assay allowing for improved identification and discrimination of chemokine receptor antagonists from non-specific blockers.
Claims22
A method, comprising the steps of:
- providing an apparatus comprising an upper chamber and a lower chamber separated by a porous membrane;
- placing cells bearing a chemoattractant receptor in the upper chamber in the presence or in the absence of a candidate antagonist;
- placing an inhibitory concentration of a ligand for the chemoattractant receptor in the lower chamber;
- and
- measuring migration of cells from the upper chamber to the lower chamber,
- wherein an increased migration of cells to the lower chamber in the presence of the candidate antagonist as compared to the migration of cells in the absence of the candidate antagonist identifies the candidate antagonist as an antagonist to the chemoattractant receptor.
3 more elements
Dependencies are inferred from the claim text.