Binding assay employing human T2R taste receptors
AssigneeSenomyx, Inc.
InventorJon Elliot Adler
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Abstract
Newly identified mammalian taste-cell-specific G Protein-Coupled Receptors and the genes encoding said receptors are described. Specifically, T2R taste G Protein-Coupled Receptors that are believed to be involved in bitter taste sensation, and the genes encoding the same, are described, along with methods for isolating such genes and for isolating and expressing such receptors. Methods for representing taste perception of a particular tastant in a mammal are also described, as are methods for generating a novel molecules or combinations of molecules that elicit a predetermined taste perception in a mammal, and methods for simulating one or more tastes.
Claims47
An in vitro method for identifying a compound that putatively elicits or modulates taste in a human subject based on its effect on the activation of a taste receptor comprising a human T2R polypeptide comprising:
- (i) screening one or more compounds in a functional assay that detects compounds which activate or modulate (enhance or inhibit) the activation of a taste receptor comprising a human T2R polypeptide having the amino acid sequence in SEQ. ID. NO:2;
- or
- (b) a T2R polypeptide that possesses at least 95% sequence identity to the human T2R polypeptide of SEQ. ID. NO:
- 2 and which T2R polypeptide is specifically activated or the activation of said T2R polypeptide is modulated by a bitter ligand that modulates the activation of the human T2R polypeptide of SEQ ID NO:2
- (ii) identifying compounds (i) that putatively elicit or modulate T2R polypeptide-associated taste subject based on their (a) activation or modulation (inhibition or enhancement) of the activation of said T2R polypeptide according to (a), or (b), in said functional assay (i).
The method of 41, wherein said functional assay screens a library of compounds.
Dependencies are inferred from the claim text.