US 7,396,647 B2 · Site- or sequence-specific process for cleaving analytes and library of analytes

Abstract

This invention provides for compositions for use in real time nucleic acid detection processes. Such real time nucleic acid detection processes are carried out with energy transfer elements attached to nucleic acid primers, nucleotides, nucleic acid probes or nucleic acid binding agents. Real time nucleic acid detection allows for the qualitative or quantitative detection or determination of single-stranded or double-stranded nucleic acids of interest in a sample. Other processes are provided by this invention including processes for removing a portion of a homopolymeric sequence, e.g., poly A sequence or tail, from an analyte or library of analytes. Compositions useful in carrying out such removal processes are also described and provided.

Claims11

Dependent claims

A process for cleaving an analyte or a library of analytes at selected sites or sequences, said process comprising the steps of:

(a) providing:
(i) said analyte or library of analytes;
(ii) a chimeric nucleic acid construct comprising at least a first segment comprised of four or more universal bases, and a second segment wherein the 5′ end of said first segment is attached to the 3′ end of said second segment;
(iii) a restriction endonuclease;
(iv) reagents for hybridization and endonucleolytic digestion;
(b) hybridizing said composition (ii) with said analyte or library of analytes (i) to form hybrids between said second segment and said selected sites or sequences in said analyte or library of analyte (i);
and
(c) cleaving endonucleolytically said hybrids.

5 more elements

Dependencies are inferred from the claim text.

Facts

Application

11/237,467Filed 27 Sep 2005

Published

US 2006/0024738 A12 Feb 2006

Granted

8 Jul 2008

Term ends

Not estimatedThe source records don’t support an estimate.

Classification9 codes

C12Q 1/6818
Characterised by the detection means: involving interaction of two or more labels, e.g. resonant energy transfer
C12Q 1/6834
Hybridisation assays: enzymatic or biochemical coupling of nucleic acids to a solid phase
C12Q 1/6844
Involving nucleic acids: nucleic acid amplification reactions
C12Q 1/6853
Nucleic acid amplification reactions: using modified primers or templates

Site- or sequence-specific process for cleaving analytes and library of analytes

Life of this patent

Site- or sequence-specific process for cleaving analytes and library of analytes

Life of this patent

Abstract

Claims11