Surface-attached polyfunctional polymer networks for sensor chips

Process for the formation of a polyfunctional polymer network covalently attached to a sensor surface, wherein said network comprises cross-linked polymer subchains having a multitude of identical or different repeating units carrying one or more functional groups which allow an interaction of the polymer with sample or probe molecules, the process comprising the steps of:

• (a) covering the sensor surface with a monolayer of a polymerization initiator which comprises one or more functional groups suitable for covalent attachment to the surface, wherein said initiator comprises a chlorosilane, a disulphide, or a thiol group;
• (b) contacting the polymerization initiator monolayer with a polyfunctional polymer network comprising cross linked polymer subchains, obtained by polymerization of monomers and comonomers carrying functional groups which allow an interaction with sample or probe molecules, in the presence of cross-linkers,
• wherein said contacting is followed by initiating a covalent binding reaction between the polymer network and the sensor surface-attached polymerization initiators.

Sensor surface having covalently attached thereto a polyfunctional polymer network comprising cross-linked polymer subchains having a multitude of identical or different repeating units carrying one or more functional groups which allow an interaction of the polymer with sample or probe molecules, obtained by a process comprising the steps of:

• (a) covering the sensor surface with a monolayer of a polymerization initiator which comprises one or more functional groups suitable for covalent attachment to the surface;
• (b) contacting the polymerization initiator monolayer with a solution comprising polymerization initiators, cross-linkers, and a polymerizable mixture of monomers and comonomers carrying functional groups which allow on interaction with sample or probe molecules;
• wherein said contacting is followed by simultaneously initiating and carrying out a crosslinking polymerization reaction both in solution and on the sensor surface.

Sensor surface having covalently attached thereto a polyfunctional polymer network comprising cross-linked polymer subchains having a multitude of identical or different repeating units carrying one or more functional groups which allow an interaction of the polymer with sample or probe molecules, obtained by a process comprising the steps of:

• (a) covering the sensor surface with a monolayer of a polymerization initiator which comprises one or more functional groups suitable for covalent attachment to the surface, wherein said initiator comprises a chlorosilane, a disulphide, or a thiol group;
• (b) contacting the polymerization initiator monolayer with a polyfunctional polymer network comprising cross linked polymer subchains, obtained by polymerization of monomers and comonomers carrying functional groups which allow an interaction with sample or probe molecules, in the presence of cross- linkers;
• wherein said contacting is followed by initiating a covalent binding reaction between the polymer network and the sensor surface-attached polymerization initiators.