Corneal cells expressing active agents and methods of use thereof
OwnerKeryn Anne Williams, Douglas John Coster +1inventors
InventorsKeryn Anne Williams, Douglas John Coster, Sonja Klebe
Life of this patent
Estimating the term…
Abstract
The invention relates to methods of modifying cells of corneal tissue to express an active agent, to modified corneal tissue, to vectors utilised in such methods and to methods of xeno- and allo-transplantation utilising the modified corneal tissue. The method of modifying cells of corneal tissue to express an active agent involves exposing harvested corneal tissue to an effective concentration for transfection of an expression vector which comprises a nucleotide sequence encoding for the active agent for a period sufficient to allow infection, such that cells of the corneal tissue will express the active agent.
Claims21
A method of improving corneal graft healing and/or prolonging graft survival, wherein the method comprises exposing harvested corneal tissue to an effective concentration of an expression vector which comprises a nucleotide sequence encoding for a cytokine selected from IL-10 and the P-40 component of IL-12 for a period sufficient to allow transfection, and then transplanting the corneal tissue to an eye of a recipient such that cells of said corneal tissue will express the cytokine.
Dependencies are inferred from the claim text.