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US 7,396,468 B2Granted
Methods and system for protein separation
AssigneeAgilent Technologies, Inc.
InventorsBarry Edward Boyes, James D. Martosella
Life of this patent
Estimating the term…
Abstract
The present disclosure relates to a system and method for membrane protein separation including: fractionating a membrane sample on a reversed-phase superficially porous stationary phase at a temperature of greater than or equal to about 40° C. to recover a protein greater than about 70 weight percent of the mixture of proteins.
Claims19
A method of membrane protein separation comprising:
- fractionating a membrane sample on a reversed-phase superficially porous stationary phase at a temperature of greater than or equal to about 40° C. with a gradient comprising increasing amounts of aqueous trifluoroacetic acid in an organic solvent and alternating amounts of increasing and decreasing concentrations of formic acid in an organic solvent and alternating amounts of increasing and decreasing concentrations of an alcohol to recover greater than about 70 weight percent of the fractionated membrane sample.
Dependencies are inferred from the claim text.