Mammalian host cell modified by RNAi to inhibit α 1,6-fucosyltransferase
AssigneeKyowa Hakko Kogyo Co., Ltd.
InventorsYutaka Kanda, Mitsuo Satoh, Kazuyasu Nakamura, Kazuhisa Uchida, Toyohide Shinkawa, Naoko Yamane, Emi Hosaka, Kazuya Yamano, Motoo Yamasaki, Nobuo Hanai
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Abstract
The present invention relates to a cell for the production of an antibody molecule such as an antibody useful for various diseases having high antibody-dependent cell-mediated cytotoxic activity, a fragment of the antibody and a fusion protein having the Fc region of the antibody or the like, a method for producing an antibody composition using the cell, the antibody composition and use thereof.
Claims6
An isolated mammalian host cell in which the expression of the mRNA of α1,6-fucosyltransferase is inhibited by RNA interference, wherein when a gene encoding an antibody molecule is introduced into said host cell and expressed, said host cell produces an antibody composition comprising the encoded antibody molecule which includes an Fc region comprising complex N-glycoside-linked sugar chains bound to the Fc region, said N-glycoside-linked sugar chains having reduced levels of fucose bound to N-acetylglucosamine at the reducing end, thereby producing an antibody with increased antibody-dependent cellular cytotoxicity.
Dependencies are inferred from the claim text.