Terminal-phosphate-labeled nucleotides with new linkers

A method of increasing the rate of an enzyme catalyzed nucleoside monophosphate transfer from a terminal-phosphate-labeled nucleoside polyphosphate to detect the activity of said enzyme or said terminal-phosphate-labeled nucleoside polyphosphate, said method comprising:

• a) conducting said enzyme catalyzed nucleoside monophosphate transfer from a terminal-phosphate-labeled nucleoside polyphosphate reaction in reaction buffer comprising a manganese salt, thereby increasing the rate of said reaction over the rate of said reaction in the absence of manganese;
• wherein said enzyme is a template dependent DNA polymerase,
• further wherein said reaction is conducted in a template dependent manner.