Methods and kit for diagnosing tick borne illnesses

A method for determining the presence or possibility of a Borrelia, Babesia, and Ehrlichia infection in a patient, comprising the steps of:

• a) determining a level of antibodies against peptides of infective agents from each of Borrelia, Babesia, and Ehrlichia, wherein said Borrelia peptide has the sequence shown in SEQ ID NO:
• 7, said Babesia peptide has the sequence shown in SEQ ID NO:
• 12, and said Ehrlichia peptide has the sequence shown in SEQ ID NO:
• 15 in a sample from said patient;
• b) comparing the level of antibodies determined in step a) with levels of said antibodies from at least one healthy control individual, wherein
• (i) control levels of antibodies against Borrelia, Babesia, and Ehrlichia indicate that said patient does not have the presence or possibility of Borrelia, Babesia, and Ehrlichia infection;
• (ii) levels of antibodies greater than two standard deviations above control levels against said Borrelia peptide indicate a presence or possibility of an infection with Borrelia;
• (iii) greater than two standard deviations above control levels of antibodies against peptides of Babesia indicate a presence or possibility of an infection with Babesia;
• and
• (iv) greater than two standard deviations above control levels of antibodies against peptides of Ehrlichia, indicate a presence or possibility of an infection with Ehrlichia.